The Jackson Rooming House, also known as Jackson House, is a historic building constructed in 1901 as a boarding house in the city of Tampa, in the U.S. state of Florida. It provided accommodations to African-Americans and other travelers of African descent during the era of racial segregation. It is located on the north end of downtown at 851 Zack Street, approximately one block west of Tampa Union Station. On March 7, 2007, it was added to the U.S. National Register of Historic Places.

The Jackson Rooming House was one of the only places in Tampa where black travelers could find lodging, as they were not accepted in standard hotels of the day. The 24-room establishment began as a six-room cottage built by Moses and Sarah Jackson in 1901. Soon after, they added bedrooms and a second story in order to operate the rooming house, which remained in business until 1989. The Jacksons’ children inherited the business and the home remains in the possession of one of the Jacksons’ grandchildren.